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VOLUME 3 , ISSUE 2 ( September, 2008 ) > List of Articles

Original Article

The study of the feasibility of segmental bone defect repair with tissue- engineered bone membrane: a qualitative observation

Lin Zhao, Jun-Li Zhao, Lin Wan, Shuan-Ke Wang

Keywords : Osteogenesis, Tissue engineering, Mesenchymal stem cells, Stem cell transplantation, Osteogenic membrane

Citation Information : Zhao L, Zhao J, Wan L, Wang S. The study of the feasibility of segmental bone defect repair with tissue- engineered bone membrane: a qualitative observation. 2008; 3 (2):57-64.

DOI: 10.1007/s11751-008-0034-z

License: CC BY-NC-SA 4.0

Published Online: 01-09-2008

Copyright Statement:  Copyright © 2008; Jaypee Brothers Medical Publishers (P) Ltd.


The objective of the study was to investigate the feasibility of intramembranous osteogenesis from tissue-engineered bone membrane in vivo. Bone marrow mesenchymal stem cells (MSCs) of rabbits were harvested, expanded and some of them were induced into osteoblasts. Porcine small intestinal submucosa (SIS) was converted by a series of physical and chemical procedures into a scaffold. MSCs and induced osteoblasts were seeded separately onto the scaffold, thus fabricating two kinds of tissue-engineered bone membrane. A total of 12 New Zealand rabbits were subjected to a surgical operation; a 15 mm bone segment, including the periosteum, was resected from the radius on both sides of each rabbit to create critical bone defects. The two kinds of tissue-engineered bone membrane and SIS (as control) were implanted randomly into the site of bone defect. The animals had radiographs and were killed after 4 weeks. The specimens were harvested and histological examination performed for evidence of osteogenesis. Bone tissue had formed in defects treated by the two kinds of tissue-engineered bone membrane at 4 weeks. This was supported by the X-ray and histological examination, which confirmed the segmental gap bridged by bone. There was no attempt to bridge in the bone defect treated by SIS. Tissue-engineered bone membrane, constructed by seeding allogeneic cells on an xenogeneic and bio-derived scaffold, can repair critical bone defects successfully.

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  1. Olivier G, Ralph M, Dietrich von S et al (2005) In vivo bone regeneration with injectable calcium phosphate biomaterial: a three-dimensional micro-computed tomographic, biomechanical and SEM study. Biomaterials 26:5444-5453
  2. Parikh SN (2002) Bone graft substitutes: past, present, future. J Postgrad Med 48:142-148
  3. Petite H, Viateau V, Bensaid W et al (2000) Tissue-engineered bone regeneration. Nat Biotechnol 18:959-963
  4. Quarto R, M astrogiacomo M, Cancedda R et al (2001) Repair of large bone defects with the use of autologous bone marrow stromal cells. N Engl J Med 344(5):385-386
  5. Shang Q, Wang Z, Liu W, Shi Y, Cui L, Cao Y (2001) Tissueengineered bone repair of sheep cranial defects with autologous bone marrow stromal cells. J Craniofac Surg 6:586-593
  6. Shakesheff K, Cannizzaro S, Langer R (1998) Creating biomimetic microenvironments with synthetic polymer-peptide hybrid molecules. J Biomater Sci Polym Ed 9(5):507-518
  7. Ishaug SL, Crane GM, Miller MJ, Yasko AW, Yaszemski MJ, Mikos AG (1997) Bone formation by three-dimensional stromal osteoblast culture in biodegradable polymer scaffolds. J Biomed Mater Res 36(1):17-28
  8. Kotobuki N, Hirose M, Takakura Y, Ohgushi H (2004) Cultured autologous human cells for hard tissue regeneration: preparation and characterization of mesenchymal stem cells from bone marrow. Artif Organs 28:33-39
  9. Turhani D, Watzinger E, Weissenbock M, Cvikl B, Thurnher D, Wittwer G, Yerit K, Ewers R (2005) Analysis of cell-seeded 3-dimensional bone constructs manufactured in vitro with hydroxyapatite granules obtained from red algae. J Oral Maxillofac Surg 63(5):673-681
  10. Turhani D, Watzinger E, Weissenbock M, Yerit K, Cvikl B, Ewers R, Thurnher D (2005) Expression pattern of the chromosome 21 transcription factor Ets2 in cell-seeded three-dimensional bone constructs. J Biomed Mater Res A 73(4):445-455
  11. Freed LE, Vunjak-Novakovic G (1998) Culture of organized cell communities. Adv Drug Deliv Rev 33:15
  12. Hua Ye, Diganta B. Das, James T. Triffitt, et al (2006) Modelling nutrient transport in hollow fibre membrane bioreactors for growing three-dimensional bone tissue. J Membr Sci 272:169-178
  13. Griffith LG, Naughton G (2002) Tissue engineering—current challenges and expanding opportunities. Science 295:1009
  14. Mizuno H, Hata K, Kojima K, Bonassar LJ, Vacanti CA, Ueda M (2006) A novel approach to regenerating periodontal tissue by grafting autologous cultured periosteum. Tissue Eng 12(5):1227- 1335
  15. Vogelin E, Jones NF, Huang JI, Brekke JH, Lieberman JR (2005) Healing of a critical-sized defect in the rat femur with use of a vascularized periosteal flap, a biodegradable matrix, and bone morphogenetic protein. J Bone Joint Surg Am 87(6):1323-1331
  16. Kanou M, Ueno T, Kagawa T, Fujii T, Sakata Y, Ishida N, Fukunaga J, Sugahara T (2005) Osteogenic potential of primed periosteum graft in the rat calvarial model. Ann Plast Surg 54(1):71-78
  17. Hattori K, Yoshikawa T, Takakura Y, Aoki H, Sonobe M, Tomita N (2005) Bio-artificial periosteum for severe open fracture—an experimental study of osteogenic cell/collagen sponge composite as a bio-artificial periosteum. Biomed Mater Eng 15(3):127-136
  18. Aggarwal S, Pittenger MF (2005) Human mesenchymal stem cells modulate allogeneic immune cell responses. Blood 105:1815-1822
  19. Pittenger MF, Mackay AM, Beck SC et al (1999) Multilineage potential of adult human mesenchymal stem cells. Science 284:143-147
  20. Vats A, Tolley NS, Buttery LDK, Polak JM (2004) The stem cell in orthopaedic surgery. J Bone Joint Surg 86-B(2):159-64
  21. Horwitz EM, Le Blanc K, Dominici M, Mueller I, Slaper- Cortenbach I, Marini FC et al (2005) Clarification of the nomenclature for MSC: the International Society for Cellular Therapy position statement. Cytotherapy 7(5):393-395
  22. Le Blanc K, Tammik L, Sundberg B et al (2003) Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex. Scand J Immunol 57(1):11-20
  23. Gotherstrom C, Ringden O, Tammik C et al (2004) Immunologic properties of human fetal mesenchymal stem cells. Am J Obstet Gynecol 190(1):239-245
  24. Julius A (2003) Potian, Hana Aviv. Veto-like activity of mesenchymal stem cells: functional discrimination between cellular responses to alloantigens and recall antigens. J Immunol 171:3426- 3434
  25. Allman AJ, Mcpherson TB, Badylak SF et al (2001) Xenogeneic extracellular matrix grafts elicit a Th2-resistricted immune response. Transplantation 71(11):1631
  26. Hodde JP, Record R, Liang HA et al (2001) Vascular endothelial growth factor in porcine-derived extracellular matrix. Endothelium 8(1):11
  27. Badylak SF, Liang HA, Record R et al (1999) Endothelial cell adherence to small intestinal submucosa: an acellular bioscaffold. Biomaterials 20(23-24):2257
  28. Ishikawa K, Ueyama Y, Mano T et al (1999) Self-setting barrier membrane for guided tissue regeneration method: initial evaluation of alginate membrane made with sodium alginate and calcium chloride aqueous solutions. J Biomed Mater Res 47(2):111-115
  29. Meinig RP (2002) Polylactide membranes in the treatment of segmental diaphyseal defects: animal model experiments in the rabbit raius, sheep tibia, Yucatan minipig radius, and goat tibia. Injury 33(Suppl 2):B-58-B-65
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